ALKBH5 Knockout cell line (HCT 116)

ALKBH5 Knockout cell line (HCT 116) Order

Catalog#

YKO-HS3275

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ALKBH5 Knockout cell line (HCT 116)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

ALKBH5

Gene ID

54890

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 1 (based on transcript ALKBH5-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HCT 116			STR Info (Cell bank) Cell Line:HCT 116
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	11		10	11
D13S317	10	12		10	12
D7S820	11	12		11	12
D16S539	11	13		11	13
VWA	17	22		17	17
TH01	8	9		8	9
AMEL	X	X		X	Y
TPOX	8	8		8	9
CSF1PO	7	10		7	10
D12S391	17	21
FGA	18	23
D2S1338	16	16
D21S11	29	30
D18S51	16	17
D8S1179	12	14
D3S1358	12	19
D6S1043	13	13
PENTAE	13	14
D19S433	12	13
PENTAD	9	13
D1S1656	12	14

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ALKBH5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ALKBH5 gene knockout ALKBH5 Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ALKBH5 Knockout cell line (HCT 116)

Catalog#

YKO-HS3275

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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